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By using fusions of the well-understood Tn21 mercury resistance operon (mer) with promoterless luxCDABE from Vibrio fischeri, we have constructed and tested three biosensors for Hg(II). SPs share some risk factors with conventional adenomas and have been associated with tobacco use, obesity, and age. In basement membrane surrounding existing blood vessels, endothelial cells stimulated in this way can presumably migrate through the resulting gaps and form new vascular sprouts.

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Antibiotic resistance caused by gram-negative multidrug efflux pumps. Responses of pericruciate cortex neurons to stimulation of the hypothalamus and reticular formation Suppression of human cytotoxic T lymphocyte responses by adherent peripheral blood leukocytes. Virus strains that were typed during the 2002 epidemic show that DENV-3 has displaced other dengue virus serotypes and entered new areas, a finding that warrants closer evaluation. This study presents survey data on low-income elderly people who attended daytime meal programs.

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monocytogenes-infected rats, UV-B exposure caused an increased number of bacteria in the spleen, coupled to a decreased specific response of T lymphocytes to the bacteria. Transient myocardial ischemia occurring in patients with unstable angina is associated with an adverse prognosis. During the course of this study a proteolytically inactive mutant of NS3 was characterized carrying a previously unreported amino acid substitution near the proposed active site of the enzyme. Saline-treated controls displayed a stable exploratory behaviour, declining during the time period measured. There was no effect on the P 190-amplitude whereas the N 110-amplitude shows distinct responses on changes in GAL.

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The molecular mechanisms underlying the TFF1 tumor suppressor functions remain unclear. Microtubules cross-linked by the kinase became partly resistant to the depolymerizing action of vinblastine. Chronic unpredictable stress (CUS) has been shown to augment the increase of dopamine in the striatum when challenged with high doses of methamphetamine immediately following stress exposure, however it is not known whether this neurochemical stress-sensitization continues after the cessation of the stressors or if behavioral sensitization is also present. A controlled sequential trial was performed comparing the efficacy of three treatments to untreated controls when applied 1 hr after subcutaneous injection of rats with Crotalus atrox venom.

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Microsatellite analysis of eggs revealed that workers laid eggs in more than half the queenright experimental nests. The addition of autologous erythrocytes to a mixture of human peripheral blood lymphocytes and sheep red blood cells (SRBC) alters the spontaneous rosetting reaction in the test detecting the active fraction of T lymphocytes (ARFC). The number of colorectal cancer deaths and the population for each 5-year age group were obtained from Statistics Korea for the period 1984-2013 for adults 30 years and older. After the reaction with the immunoglobulin fraction glycogen preparations were less effectively degraded by alpha-amylase.

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Experiments using dominant-negative Tpl2 suggest this kinase functions distal to TRAFs but proximal to the TAK1/TAB1 signaling complex, within the IKK/NFkappaB activation pathway. No patient exhibited clinical muscle disease without electrocardiographic atrial disease. Patients with stable chronic obstructive pulmonary disease can safely undergo intravenous dipyridamole thallium-201 imaging. HLA-A and HLA-B markers have been determined in fibroblasts grown from tissues of triploid conceptuses and have been tested in the parents. Distribution of basement membrane proteins in normal and fibrotic human liver: collagen type IV, laminin, and fibronectin.

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Furthermore, a monoclonal anti-B-cell-stimulating factor-1 antibody only partially blocked the fibroblast proliferation induced by T-cell supernatants. The large polygonal cells characteristic of the adult bladder begin to appear by day 15, but the microridges are not apparent until day 17.

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Safety of alternating ganciclovir and foscarnet maintenance therapy in human immunodeficiency virus (HIV)-related cytomegalovirus infections. Cloning of selective high absorbance wells were done with limiting dilution method. Only antibodies to IFN-alpha/beta were able to abrogate the protective effects of HMA alginate in macrophages infected with HSV-1, suggesting that the antiviral activity induced by this immunomodulator was mediated by the production of IFN-beta. The I-V delay is increased in retro-cochlear hearing loss and not different of the normal in cochlear hearing loss. Ultrastructural study of the development of the pars distalis (anterior pituitary) in the foal.

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Monitoring and blunting were assessed with the Miller Behavioural Style Scale (MBSS) at two points in time: before and after (i.e. The use of transgenic mice that report on toxic insult-induced transcription can provide a valuable tool in this regard. We conducted a blinded data review of 45,416 encounters obtained from a prospectively collected database enrolling adult patients admitted or observed with the following inclusion criteria: (1) primary presenting symptom of chest pain, chest tightness, chest burning, or chest pressure and (2) negative findings for serial biomarkers.

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Capillary method for assessment of pulmonary maturity in utero with the use of amniotic fluid. Stone-formers had a lower concentration of citrate in their urine than either of the control groups. Several clinical parameters showed that the 21 patients whose strains constituted the main cluster, were in worse clinical condition than the other patients in the study group. Radiographic evaluation was performed by magnetic resonance imaging (MRI) obtained pre-operatively and at 6 and 12 weeks post-operatively. Consensus interferon and ribavirin in patients with chronic hepatitis C who were nonresponders to pegylated interferon alfa-2b and ribavirin.

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Addition of serotonin (final concentration 0.06, 0.6 and 6.0 uM) stimulated the release of LH and FSH into the perifusion fluid draining the pituitary in series with the median eminences. The studies demonstrated that beta-naphthylamine occurs in air only at scaling of phenyl-beta-naphthylamine and its concentration does not exceed 0.0024 mg/m3.

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Because rehabilitation is important for the outcome of multiple injured patients, a further improvement of the quality and integration into the regional trauma network seem to be necessary. We have achieved very good therapeutic results with the tocolytic agent Th 1165 a (Partusisten).

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Dose-response curves were established for subcutaneous fibrosis and telangiectasia in patients with different genotypes. The coding sequence of the Haemophilus influenzae ORF I gene was amplified by PCR and cloned into different Escherichia coli expression vectors. Docking tools created for structure-based design and virtual screening have also been used to automate ligand alignment for comparative molecular field analysis (CoMFA). Burkholderia mallei and Burkholderia pseudomallei: the causative micro-organisms of glanders and melioidosis. A spontaneously beating guinea pig Langendorff heart preparation was used to evaluate and compare the effects of bepridil hydrochloride (BP) and other Ca blockers.

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Recommendations for the clinical evaluation of non-neurological female urinary incontinence Effect of maleic acid on the kinetics of alpha-methyl-D-glucoside uptake by isolated rat renal tubules.

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The Inc3B-deficient mutants isolated expressed different degrees of residual incompatibility. Adenomatous polyposis coli gene dysfunction underlies tumorigenesis in familial adenomatous polyposis patients and multiple intestinal neoplasia mice. TPO levels were significantly increased in adult patients with dengue infection in which platelets in circulation were markedly reduced, and the TPO levels were inversely related to the platelet counts.

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The complication rate including myocardial infarction, ARDS, pneumonia, and renal failure was highest (83 per cent) in those patients receiving segmental resection with a negative angiogram. Impact of image denoising on image quality, quantitative parameters and sensitivity of ultra-low-dose volume perfusion CT imaging.

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Expression of the CCN proteins is at least partly dependent on the HIF-2alpha function, the dominant HIF-alpha isoform expressed in RCC. PH-Patients are more likely to suffer from mild or subthreshold depressive syndromes, but are very interested in psychosomatic treatment. The case of a 17-year-young woman with Calcifying Fibrous Pseudotumor is reported. Depending on the desired T1, either one or a combination of the two agents is used. These results indicate that mouse tail artery is rich in postsynaptic alpha 1- and alpha 2-adrenoceptor.

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The colonocyte recovery rate was not reduced, when compared with the data for successful PCR amplification, if the fecal samples were kept at 4 degrees C after defecation and if the colonocytes were isolated within 48 hours after defecation. A double monoclonal immunoradiometric assay for factor IX antigen. Improved lentiviral transduction of human mesenchymal stem cells for therapeutic intervention in pancreatic cancer. This same region also contains the c-fos serum-responsive element.

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A number of active site residues that might be involved in this hydrogen bond are discussed. The applications of this technique are discussed and its use in evaluating lung casts is described. We have previously reported, in a group of children undergoing a single abdominal surgical procedure, the early induction of interleukin-1 receptor antagonist following the commencement of surgery. Oestrogen related side-effects were significantly lower in the 35 micrograms group during the course of the 9-month study. Detection of intracellular unimolecules using nucleic acid amplification technology

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Complete remission was obtained in 4 patients after 3 weeks and in 4 within 8 weeks of treatment. The expression of beta-2 microglobulin (beta 2m), a protein associated with the histocompatibility antigens of the pig complex (SLA), was studied in preimplantation embryos between the segmentation stage and the beginning of elongation (day 12). Cytoskeletal preparations of Trypanosoma cruzi trypomastigotes and epimastigotes contain a protein recognized by a monoclonal antibody (2G4) which is connected to the flagellar attachment zone of both stages of the parasite.

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A clinical series of 19 individuals undergoing placement of a DBS system for treatment of PD-related tremor. The determined sequence matched well the partial sequence deduced from a mitochondrial open reading frame (RF3). This effect of heparin in the presence of bile salts, in comparison with the failure of heparinized saline to induce weight loss in gallstones, is discussed.

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In order to obtain a deeper insight into this process, we have carried out a dissipative particle dynamics study of the realignment kinetics of a nanodroplet of a smectic-A liquid crystal suspended in an isotropic fluid following a switch in the direction of an applied magnetic field. Longer duration of interferon monotherapy does not appear to have a beneficial effect on the response rate.

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Quantitative Polymerase Chain Reaction to Assess Response to Treatment of Bacterial Vaginosis and Risk of Preterm Birth. An exopolysaccharide-producing microalgal dinoflagellate was isolated from a red-tide bloom and designated strain KG03.

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The parental chromosome constitution in the hybrid cells was clone-specific, even in the clones derived from the same parental cells. LLO with Cys484 is strongly connected to the surface of the cell but LLO Ala484 or Ser484 is easy removed by the washing.

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The data demonstrate that substrata can have a significant effect on maintenance and differentiation of adult neurones in primary culture.(ABSTRACT TRUNCATED AT 250 WORDS) Inverse modification of antibody responsiveness to RGG in lines of mice selected for high or low responses to somatic antigen of Salmonella. In patients exhibiting the increase of histaminemia, symptoms of hypersensitivity to aspirin, other than dyspnea, were not common and stronger.

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Aside from Gaucher disease, antibodies often lead to infusion associated reactions and a reduced biochemical response. The pericardium imposes a restraint on the interventricular septum and free walls of the ventricles during volume loading, but not during pressure loadings, because dilation of one ventricle is associated with shrinkage of the other. The potential value of integrated natriuretic peptide and echo-guided heart failure management.

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OKT8-staining was seen sporadically in the medullary cord, but there were slightly more OKT8-positive lymphocytes in the medullary cord in these spleens than in the normal spleen. These are transmembrane proteins with an extracellular binding domain that specifies the growth factor with which it will interact, and an intracellular domain that encodes the tyrosine kinase. The program was aimed at reducing back and pelvic posterior pain during pregnancy. Present areas of research include efforts to expand the donor pool through the use of umbilical cord blood and human leukocyte antigen-haploidentical donors, the use of reduced-intensity conditioning regimens, which allow treatment of previously ineligible patients, enhancement of immune reconstitution after transplantation, more effective prevention and treatment of acute and chronic graft-versus-host disease, and the augmentation of the immunologic graft-versus-tumor response and its uncoupling from deleterious graft-versus-host alloreactivity. The authors do not recommend the umbilical vein usage while portocaval anastomosis forming.

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Climate change, nevertheless, poses both opportunities and threats to the wastewater reclamation industry. Fabrication and characteristics of hexagonal Zn nanowires prepared by heating a mixture of Zn and graphite powders. Immunoblotting and immunohistochemical analysis revealed that platelets expressed WAVE-1 and WAVE-2, whereas WAVE-3 expression was hardly to be detected.

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These results support the utility of ultra high-field MRI system for noninvasive assessment of effects of trigeminal nerve injury upon the trigeminal pathways. The results suggest that implantation failure in older women largely could be due to aneuploidy. Each mammogram was assigned a grade for breast type, preference (Rh/Rh, Mo/Mo, or previous mammograms), contrast, and sharpness. The functional limitations of flaccid paralysis are the result of a permanent or progressive muscular weakness and ensuing contractures, deformities and instabilities which require compensatory and adaptive mechanisms from the patient to cope with these impairments. Rather, the sequences of alpha-amylase mRNA are rapidly degraded during heat shock as shown by in vitro translation and dot blot hybridization with a cDNA probe.

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Prednisolone and dexamethasone were of intermediate potency and ranked similar to hydrocortisone which is in contrast to their anti-inflammatory properties. The aim of this study was to evaluate outpatient, non-antibiotic management in acute uncomplicated diverticulitis with regard to admissions, complications, and recurrences, within a 3-month follow-up period. To evaluate whether the pulsatile component of blood pressure can be a risk factor independent of the steady component in elderly females. Evaluation of left ventricular function in ischemic heart disease by systolic phase structure and echocardiographic indices

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After the 3 months of treatment these patients showed a significant decrease of rT3 (P less than 0.02) and of delta-TSH (P less than 0.01). Effects of oxygen and sulphydryl-containing compounds on irradiated transforming DNA. We succeeded in isolating fetal cells from maternal blood samples in the first trimester for chromosome defects genetic diagnosis.

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It can be speculated that some inherent similarity exists in contractile processes of the phasic responses of the longitudinal muscle and contractions of the circular muscle, or that agonists act via the same (or similar) population of calcium channels. Preparation and utilization of an ion-specific calcium minielectrode. An infusion method designed to produce a gradual ramplike rise in plasma glucose levels in man showed that the observed slope of the ramp was not significantly different from the calculated slope only when both of the following parameters were used: 1. These preliminary observations are consistent with the hypothesis that AVMs and SWD represent more mature vessels, consistent with possible lesion genesis during early phases of embryonic vascular development (dysvasculogenesis).

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Unexpectedly, periplasmic export of FLAGATS-NAT1 by the ompA signal peptide of pFLAG-ATS proved to be detrimental to enzyme activity. Pharmaceutical representatives and emergency medicine residents: a national survey. Our data indicate that protein VI shuttles between the nucleus and the cytoplasm and that it links hexon to the nuclear import machinery via an importin alpha/beta-dependent mechanism. Changes in cardiac protein composition occur in a variety of patho-physiological situations and are usually accompanied by modifications in protein synthesis.

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Hydrothermal acid treatment for sugar extraction from Golenkinia sp. No certain excess of childhood cancer was seen in the most exposed areas, but three infants, in utero at the time of the accident, developed leukemia. Within the overlap zone, the subjective vertical appeared bistable on repeated testing with responses in both categories. Infants with lead poisoning showed higher saliva lead than a normal infant.

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The influence of rabies immunization of gamma-aminobutyric acid metabolism in the brains of animals Collectively, these data suggested interference with a common pathway controlling cell growth and cell cycle progression. Our aim was to study the renal function in low birth weight infants undergoing surgery with low-flow sevoflurane anesthesia.

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We demonstrate that core histones can affect the accessibility of a DNA element positioned outside of the classically defined nucleosome core region. Immune status of thalassemic patients receiving deferiprone or combined deferiprone and desferrioxamine chelation treatment. The treatment therapy for renal patients includes both dialysis (haemodialysis and continuous ambulatory peritoneal dialysis) and transplantation.

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Corresponding calculations are also performed with two widely used nonpolarizable force fields. Mastopexy alone results in an average decrease in one brassiere cup size.

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Cyanide antidotes and methods of their administration in dogs: a comparative study. Stepwise enhancement of centripetal acceleration during centrifugation induces a rapid increase in erythrocyte deformation. To meet this need, practitioners of translational medicine are developing new technologies that can facilitate decision making during the early stages of drug discovery and clinical development. Once aggressive form ATL is developed in HTLV-1 carriers, their prognosis is poor even if they receive the intensive combined chemotherapy. It also suggests that antioxidants may be a therapeutic option for preventing cardiovascular damage in diabetes mellitus in humans.

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Agreement between ultrasound and anatomical measurements of the endometrial thickness. The machined alloy Au 1.7-Ti 0.1-Ir provides sufficient bond strength to veneering ceramics, but this has to be proven by a clinical study. Intravenously injected HRP permeated the intercellular spaces of SA and reached the surface of the enamel, however it did not penetrate the distal junctions of RA.

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Cranial computed tomography of elderly patients: an evaluation of its use in acute neurological presentations. Using this method, a yeast YKE2 gene (a yeast homolog of murine k-region expressed genes) were successfully disrupted by replacement of HIS3 marker gene.