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High-speed droplet-allele-specific polymerase chain reaction for genotyping of single nucleotide polymorphisms. Intracoronary infusions of acetylcholine (5.0 and 10.7 micrograms/min) produced a dose-related increase in the subendocardial-subepicardial blood flow ratio (endo/epi) from 1.07 to 1.32 and 1.57, respectively.

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Hydroxysteroid dehydrogenases in the ovine endometrium and trophoblast. In-vitro study of the activity of ciprofloxacin alone and in combination against strains of Pseudomonas aeruginosa with multiple antibiotic resistance.

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Rapid progress has recently been made in generating monoclonal antibodies against human lymphoid cells and extensive studies in clinical medicine have defined the potential application of these monoclonal antibodies. Induction of polyploidization in leukemic cell lines and primary bone marrow by Src kinase inhibitor SU6656. An outbreak of diarrhea due to verotoxin-producing Escherichia coli in the Canadian Northwest Territories.

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Relative susceptibilities of cyclops species from Rajasthan State to guinea worm (Dracunculus medinensis) larvae. It has been validated in the analysis of asymmetric synapses of the rat hippocampal CA1 stratum radiatum affected by transient cerebral ischemia. To analyze the role of the two cleavage sites and the spacer peptide, both sites were individually mutated and a deletion mutation that precisely removes the spacer peptide was constructed. The fact that these genes are regulated by the bvg locus suggests that they play a role in the pathogenesis of the bacterium.

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Effects of endothelin-1 on capsaicin-induced nociception in mice. In the first experiment, the neurotoxic agent 6-hydroxydopamine was infused directly into the nucleus accumbens to investigate the effects of DA depletion on lever pressing performance. Unilateral carotid artery ligation in the Mongolian gerbil as a model for testing infarction-reducing therapies. At least two factors with the capacity to induce IgM synthesis in human B cells were found to be present in the 15-20-kDa fraction of the supernatant of mononuclear cells activated with concanavalin A (Con A) and phorbol ester. In surgical patients, the utility of peri-operative statin therapy is strongly suggested by retrospective studies, although it is probably overestimated, as important confounding factors have not been controlled for and hence the literature is considered to be currently inconclusive.

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Synthesis of carbocyclic nucleoside analogs with five-membered heterocyclic nucleobases. Librarians were asked to submit information for an annual architectural feature on projected, partially funded, architect selected, nearly designed, bid completed, under construction, almost finished, or recently completed library construction. 1H NMR studies of the interactions of self-associating intercalating agents with dinucleotides: a theoretical analysis. The objective of this study was to analyze the exact anatomic data collected from a large group of patients undergoing total knee arthroplasty and to correlate the measurements to the dimensions of current prosthetic systems. high nitrate exposure groups, showing development of hypertrophy at nitrate levels exceeding 50 mg/l.

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In NOD mice, an animal model of IDDM, the T cell receptor (TCR) of the infiltrating T lymphocytes was reported to be variable. Ophthalmologic emergency caused by the skin secretion of a scabrous toad The reactivities of these MAbs (5C3 and 3C8) against mouse and human host cells were studied by Western blotting and flow cytometric analysis. Disinhibition of these neurons could lead to seizure suppression and may be involved in the nigral control of epilepsy.

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Late cyclosporine treatment ameliorates established coronary graft disease in rat allografts. 20 days after a freeze and thaw cycle only the spectrometry of the colony size and survival curve of the cells and colonies suggest a cell lesion. The death rate of injected labelled cells is followed by measuring the appearance of label in the urine.

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Kinetic and equilibrium studies of the reactions of heme-substituted horse heart myoglobins with oxygen and carbon monoxide. These data are not consistent with either a sodium or an osmoreceptor mechanism located within the blood-brain barrier (BBB) or with a peripheral sodium receptor mechanism. Mice were immunized and hybridomas were prepared by fusing spleen cells with Sp2/0-Ag14 myeloma cells using poly(ethylene glycol). Participant research: an alternative pedagogical methodology for nurses

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These results support the hypothesis that iron affects pancreatic islet cell function and may be an etiologic agent of diabetes mellitus in hemochromatosis. Cloning and expression in Escherichia coli of the gene encoding beta-C-4-oxygenase, that converts beta-carotene to the ketocarotenoid canthaxanthin in Haematococcus pluvialis. First, the individual parent axons showed unrestrained trajectories within the main bundle which constantly occupied the ventral part of the dorsal column. The complement system is a major constituent of the innate immune system. This combination of respiratory alkalosis and metabolic acidosis resulted in normalization of the pH at about 18 minutes of fibrillation.

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In this paper, we show the above coding scheme to be inefficient in various ways and improve upon it so that it can compress DNA. A 2-year follow-up pilot study evaluating the safety and efficacy of op-1 putty (rhbmp-7) as an adjunct to iliac crest autograft in posterolateral lumbar fusions. Percutaneous transluminal coronary angioplasty was successfully performed. Three decision-making patterns--straightforward, moderately straightforward, and complex were identified as well as the factors that influenced the process. Retention of Pediatric Advanced Life Support (PALS) course concepts.

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Popular and unpopular infectious agents linked to primary biliary cirrhosis. Their in vitro antiproliferation activities were also evaluated.

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Spermine partially reaggregates the bound polysome and a combination of spermine and arginine augments albumin synthesis to the control rate. Relationship of environmental tobacco smoke to otitis media in young children. Thus, this mature B cell line secretes two distinct antibody molecules but is allelically excluded at the level of surface Ig expression. Both DR and MT class II HLA molecules may restrict proliferative T-lymphocyte responses to antigen. Mitochondria are key players in the maintenance of cellular homeostasis, as they generate ATP via OXPHOS.

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The Bacterial Sec Pathway of Protein Export: Screening and Follow-Up. This suggests that epitopes spatially located near the recognition site of apoB for cellular receptors have a greater tendency to be conserved. The simulations demonstrate that there is great potential for the production of functional materials with high overheating protection efficiency. How many testers are needed to assure the usability of medical devices? Second, MG-63-derived HPGF purified to electrophoretic homogeneity was subjected to amino acid sequence analysis, whereby the NH2-terminal sequence was found to match the nucleotide sequence of a 26 kD cDNA clone.

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Respiration during sleep was monitored in ten patients with chronic obstructive pulmonary disease (COPD) and related to measures of hemoglobin saturation and intrathoracic pressure (ITP) or abdominal wall movement. The distribution coefficients of N-acetyl ethyl esters of glycine, diglycine, beta-alanine, alanine, valine, norvaline, leucine and norleucine between water and different organic solvents have been measured at different temperatures.

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The data show that cholinergic and nitrergic neurotransmission only occurs in the gastric longitudinal layer in regions where ICC-IM are present. This interference which is observed at least during the 24 hours following intravenous administration of noramidopyrine, results in either an increase (Folin-Denis) or a decrease (uricase) of the intensity of the final colour. Increased frequency of sister chromatid exchanges in lymphocytes of Chernobyl clean-up workers. A PAOP-LAP gradient may develop during acute respiratory failure.

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The BST-2 gene (HGMW-approved symbol BST2) is located on chromosome 19p13.2. If a structure is established that allows participation in decision-making and mutual adjustment, trained professionals can perform up to their standards and those standards of their peers. Study of the mechanism of metabolic activation of chloramphenicol by rat liver microsomes.

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As part of the investigative method, we have designed a clinical protocol adapted to the needs of the study, in this respect, we have used gastrointestinal intubation to obtain independent samples of gastric and duodenal juices, by means of a long nasoduodenal tube or a modified Dreimlich tube, placed manually in the intraoperative period, during the cholecystectomy. Outcome variables analyzed included upper esophageal sphincter pressure (UESP), upper esophageal sphincter residual pressure (UESRP), lower esophageal sphincter pressure (LESP), lower esophageal sphincter residual pressure (LESRP) and esophageal body peristalsis.

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Participants were administered the Schedule for the Assessment of Negative Symptoms (SANS), the Positive and Negative Symptom Scale (PANSS), and the Calgary Depression Scale for Schizophrenia. A great deal of investigation is still needed in developing assays for detecting phototoxic chemicals and for defining mechanisms involved in phototoxic events in human skin. The viral cores which accumulate in chick embryo fibroblasts in the presence of cycloheximide are further uncoated after removal of the protein synthesis inhibitor. Conditions and times for the formation of type A enterotoxin in confectionery cream products Continuity of care prism process applied to the congestive heart failure population.

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Western-blot and Northern-blot analysis indicated that mice either lacking or overexpressing PrPc had levels of Cu,Zn SOD mRNA equivalent to those expressed in wild-type mice. Three-dimensional flame structures and temperature distributions in the flame interior were visualized using the visualization tool kit. Early-onset Behr syndrome due to compound heterozygous mutations in OPA1. Reconstructing the evolution of vertebrate blood coagulation from a consideration of the amino acid sequences of clotting proteins.

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Relationship between thrombopoietin serum levels and liver function in patients with chronic liver disease related to hepatitis C virus infection. Initial analysis of the data indicated an anisotropic aquifer system on the basis of the observed radial and tangential deformations. The vibrations of the malleus measured with this instrument turn out to be in excellent agreement with data by previous workers.

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Because of the extensive array of enzymes in biological cells, we also develop three new hypotheses: that enzymatic self diffusion can assist in organizing signaling pathways in cells, can assist in pumping of fluid in cells, and can impose biologically significant forces on organelles, which will be manifested as stochastic motion not explained by thermal forces or myosin II. The charge delocalised beta,beta-carotene dication--preparation, structure elucidation by NMR and reactions with nucleophiles. The drug used in the course treatment of patients accelerates the normalization of cerebral vascular responsiveness at the site of surgical intervention. The bar was surgically removed and the postoperative course was unremarkable.

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Clinical staff were highly motivated to implement the guideline but hindered by organizational constraints. Either of these selected motifs was able to restore ETR-3 binding and responsiveness to a nonresponsive splicing reporter in vivo. Instead, mitochondrial carbamoyl phosphate accumulated over 40-fold and turned over rapidly. Then there was a bilateral oculomotor palsy involving both external recti, and exophthalmos.

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Except for a modest increase in systolic blood pressure at peak exercise during ranolazine therapy, hemodynamic measurements did not differ significantly during ranolazine and placebo therapies. Importance of technical variables for quantitative measurements by color Doppler imaging. We estimated outcomes from 3-month follow-up data using logistic and ordinary least squares regression. The two recombinants differed in truncation of 26 and 71 amino acids, respectively, in the carboxy-terminal sequence of DEN-specific envelope (E) glycoprotein.

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New drug combinations against falciparum malaria which are both effective and affordable for Sub-Saharan African populations are urgently needed. Genomic DNA was prepared from white blood cells, and LDLR and PCSK9 mutations were identified using the Invader assay method. From May 2003 to April 2004, 30 patients with moderate or total urinary incontinence, following radical prostatectomy or endoscopic resection of the prostate, underwent the new technique. Furthermore, the hypothyroid state, which was characterized by a markedly diminished 125iodine incorporation into the thyroid, no body weight gain and undetectable serum thyroxin concentrations, was compensated by the grafted tissue. Suspicion of this situation is asymptomatic patients with pneumoperitoneum and a rigorous clinical check can avoid unnecessary laparotomies.

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The pColV-K30-978-77 chimeric gene encoded a 76-kDa outer membrane protein which mediated a low level of cloacin susceptibility, whereas the 978-77-pColV-K30 type encoded a protein of 74 kDa determining a level of cloacin susceptibility identical to that mediated by pColV-K30. The number of circulating T-lymphocytes and their functional activity were studied in a group of 14 uremic patients prior to and after 6 to 9 months of treatment with continuous ambulatory peritoneal dialysis (CAPD). The pregnant female differs from the non-pregnant in several features, which appear to be due to the presence of the conceptus in the uterus. Despite this, only one patient reported resolution of symptoms, three patients had reduced straining, and three patients had some gain in insight.

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The effects on the electric field due to coil rotation, in combination with target site anatomy, have been quantified. Clinical value of the determination of drugs in the blood: a new aspect of pharmacokinetics Parietal thrombosis and leukocyte diapedesis were also observed in the aorta.

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Activity-initiated rate-responsive ventricular pacing resulted in a fourfold greater increase in cardiac output with exercise compared to fixed rate pacing. The data show that EDRF mRNA levels in healthy persons vary within a total range of 2 log units as well as they display a weak correlation with body height. No relationship was seen between the levels of sCD23 and IFN-gamma and allergy.

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Interaction between opioid peptides and monoamines in the regulation of gonadotropin secretion in the female rat. Within two respectively three years after endometrial ablation, two other patients died from causes unrelated to the surgical intervention (cardiac infarction, cerebral haemorrhage).

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These differences could not be explained by age, direct neuroleptic effect, or neuroleptic exposure time. Isolates of caprine arthritis-encephalitis virus recovered 12 to 18 months after infection of goats were tested for neutralization by using rabbit antisera directed to the original inoculum virus.

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The phagocytic activity as revealed by latex ingestion and cytochemically demonstrated peroxidase activity of neutrophils from peripheral blood was investigated in 26 patients with pulmonary cancer, 22 patients with gastric cancer and 8 patients with tumours of large intestine and compared with results obtained in 40 healthy individuals. Bacteremic infection and malnutrition in patients with solid tumors: investigation of host defense mechanisms. Data from animals and from man suggest that calcium antagonists interfere with alpha-adrenergic receptors and that this mechanism may be responsible for some of the vasodilation induced by these drugs. A highly significant positive correlation was observed between Hg concentrations in hair and kidney cortex.

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This study examined the role of coping in explaining health-related quality of life (HRQoL) in cystic fibrosis (CF). The beneficial effects of treatments such as panretinal photocoagulation and vitrectomy on the retinal hemodynamics are shown to agree with the hypothetical model.

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Coincident with this emphasis must be the development of functional assessments to be used when documenting intervention outcomes. It is argued that the concept of diagnosis is the core of the medical model, and that psychiatry is a branch of medicine, and not a social science, because the biological aspect is very important. The present study compares the family power structure and parent-child relationship quality in delinquent and non-delinquent young subjects in Tehran. A protein containing a nucleotidyltransferase motif characteristic of poly(A) polymerases has been proposed to polyadenylate RNA in Streptomyces coelicolor (P.

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XXYY chromosomal constitution recognised during the 1st year of life Reversal by methylene blue of tetanic fade induced in cats by nitric oxide. A comparison of three antibiotic regimens for eradication of Haemophilus influenzae type b from the pharynx of infants and children. We previously reported five families with primary, genetic (idiopathic) hemochromatosis in whom HLA typing of subjects indicated that a homozygous-heterozygous mating had almost certainly occurred and in whom inheritance of the disease trait was best explained by an autosomal recessive mode of inheritance. SAS is useful for three-dimensional delineation of the brain surface before surgery.

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Injection of antidepressants during NA infusion resulted in more pronounced depressor and tachycardic effects than occurred without NA infusion. Acne conglobata: personality and psychological sequelae in 13-cis-retinoic acid therapy. Addition of salts induces a partial transition to a beta structure. Modifications of cardiac activity in the rat and guinea pig by ouabain and magnesium in a low potassium medium Determining whether dominance or overdominance is the major genomic mode is important for two long-standing evolutionary genetics issues: 1.

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Ion concentrations (Na, K and Ca) of parasympathetically nerve-evoked parotid saliva were not altered. The formation of hydrogen bond was investigated in terms of the charge density by the NBO analysis.